Subcellular marine microscopy

I am Marie Walde, a biophysicist studying small eukaryotes in the marine plankton with advanced 3D light microscopy and image analysis at the marine reserach station in Roscoff/Bretagne (France).

My research is funded by the German Research Council (Deutsche Forschungsgemeinschaft)

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About Me

I work on deciphering the morphologies and cellular interactions of unicellular marine microbes, in particular understudied small protists.
Towards this goal, I develop and use tailored bioimaging and image processing methods, including automated and super-resolution microscopy to address their challenging diversity and connect information across large scale ranges.

Career

Postdoctoral Researcher

Roscoff Culture Collection, France Jan 2025–Nov 2026

EU project BlueRemediomics: Research on marine microbial ressources

Walter Benjamin Fellow, DFG

Station Biologique de Roscoff & IFREMER Plouzané, France Sep 2021–Nov 2024

Independent Research Fellowship by the German Reserach Foundation (DFG)
Project SymbioScope: Assessing the ecological diversity and dynamics of symbiotic interactions in a key phytoplankton by automated high-content 3D microscopy

Visiting Scientist

Uppsala Universitet, Sweden Jul 2021–Aug 2021

Hosted by Fabien Burki, Department of Organismal Biology
Automated microscopy to elucidate unknown eukaryotic diversity

Postdoctoral Fellow

Station Biologique de Roscoff, France Dec 2019–Jun 2021

Stratégie d'attractivité durable (SAD) International Postdoctoral Fellowship by the Région Bretagne: Competitive fellowship aimed at international researchers who bring new methods and expertise to research establishments based in Bretagne
Supervised by Nathalie Simon & Colomban de Vargas, Ecology of Marine Plankton Group
Project IMASTAN: Automated imaging of phytoplankton in the SOMLIT-Astan time series

Imaging Scientist

Max Delbrück Center, Berlin, Germany Jan 2017–Nov 2019

Systems Biology Imaging Platform, headed by Andrew Woehler
Max Delbrück Center (MDC) for Molecular Medicine in the Helmholtz Association
Plasticity of hippocampal neurons; superresolution fluorescence neuroimaging
Host of monthly MicroscopyClub together with Nikita Vladimirov

Research & Teaching Assistant

Friedrich-Schiller-Universität Jena, Germany Nov 2010–Nov 2016

Education

PhD in Biomedical Imaging & Microscopy

Friedrich-Schiller-Universität Jena, Germany 2017 magna cum laude

Leibniz Institute of Photonic Technologies & Institute of Physical Chemistry
Supervised by Rainer Heintzmann, Nanobiophotonics Research Group
Research Project: Development of a holoscopic imaging system and applied high-resolution fluorescence microscopy

Master of Research in Molecular Biophysics

King's College London, UK 2010 Pass with Distinction

Randall Centre for Cell & Molecular Biophysics
Research Project: High-resolution fluorescence microscopy with photoswitchable proteins

Bachelor of Science in Biophysics & Physics

Humboldt-Universität zu Berlin, Germany 2009

Research Project: Resolution improvement with structured illumination fluorescence microscopy applied to biological research

Experimental Methods

Fluorescence microscopy, automated microscopy, super-resolution microscopy, image processing & quantitative analysis, plankton sampling and filtration, plankton culturing, optical design & calibration, instrument control, fluorescent labelling & immunocytochemistry, in-situ hybridization, interferometry, 3D modelling and printing

Research

Publications

  • C.W.H. Sim , M. Walde, H. Strindberg, A.Kaur, S. Le Panse, P. Gourvil, W. Eikren, D. Vaulot & A. Lopes dos Santos. A novel Labyrinthulid Labyrinthula merlionensis n. sp. and its parasitic interaction with marine diatoms.
    Manuscript in preparation, full draft on FigShare

  • J. Szymczak, S. Pinaud, I. Romero Rodriguez, M. Walde, E. Kayal, C. Alves-de-Souza, E. Bigeard, M. Gachenot, C. Jauzein, M. Le Gac, J. Decelle, S. Le Panse, G. Pohnert, M. Vallet, T. Arthur, L. Guillou. Marine Alveolates’ Dual Pathways : Exploring Infectious and Sexual Spores in the parasite Amoebophrya.
    Manuscript in preparation.

  • M. Walde, C. Camplong, C. de Vargas, A.-C. Baudoux & N. Simon (2023). Viral infection impacts the 3D subcellular structure of the abundant marine diatom Guinardia delicatula. Frontiers in Marine Science 9, 1034235.
    DOI: 10.3389/fmars.2022.1034235

  • M. M. Sandin & M. Walde (2022). oligoN-design: First beta version. GitHub Releases.
    Bioinformatic pipeline for the high-throughput design of specific probes for fluorescence in situ hybridization of only genetically known organisms.
    DOI: 10.5281/zenodo.7473220.

  • Z. Farsi, M. Walde, A. E. Klementowicz, F. Paraskevopoulou & A. Woehler (2021). Single synapse glutamate imaging reveals multiple levels of release mode regulation in mammalian synapses. iScience, 24(1), 101909.
    DOI: 10.1016/j.isci.2020.101909

  • M. Walde & A. Woehler (2018). Spectral STORM - Multi-colour super-resolution imaging with a single excitation laser. European Light Microscopy Initiative (ELMI2018), Dublin.
    DOI: 10.5281/zenodo.3833716

  • M. Walde, A. Jost, K. Wicker & R. Heintzmann (2017). Engineering an achromatic Bessel beam using a phase-only spatial light modulator and an iterative Fourier transformation algorithm. Optics Communications, 383, 64–68.
    DOI: 10.1016/j.optcom.2016.08.050

  • M. Walde (2017): Development of a holoscopic imaging system and applied high-resolution fluorescence microscopy. Friedrich-Schiller-Universität Jena. PDF

  • Badgaa, R. Büchler, N. Wielsch, M. Walde, R. Heintzmann, Y. Pauchet, A. Svatos, K. Ploss & W. Boland (2015). The Green Gut: Chlorophyll Degradation in the Gut of Spodoptera littoralis. Journal of Chemical Ecology, 41(11), 965–974.
    DOI :10.1007/s10886-015-0636-0 PDF

  • M. Walde, J. Monypenny, R. Heintzmann, G.E. Jones & S. Cox (2014). Vinculin binding angle in podosomes revealed by high resolution microscopy. PLoS ONE, 9(2).
    DOI: 10.1371/journal.pone.0088251

  • K. Wicker, M. Walde, E. Oldewurtel, L. Hirvonen, O Mandula, S. Sindbert & R. Heintzmann (2010). Structured Illumination and Image Inversion Interferometry. Biophysical Journal, 98(3), 619a.

Say Hello

Ecology of Marine Plankton
Station Biologique de Roscoff
CNRS & Sorbonne Université
Place Georges Tessier, 29680 Roscoff, FRANCE

Email

marie.walde@sb-roscoff.fr

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